Modafinil is a wake promoting drug approved for clinical use and also has cognitive enhancing properties.Its enantiomer R-Modafinil (R-MO) is not Clothing - Mens Bottoms well studied in regard to cognitive enhancing properties.Hence we studied its effect in a spatial memory paradigm and its possible effects on dentate gyrus long-term potentiation (DG-LTP).
Clinically relevant doses of R-MO, vehicle dimethyl sulfoxide (DMSO) or saline were administered for three days during the hole-board test and in in vivo DG-LTP.Synaptic levels of dopamine receptors D1R, D2R, dopamine transporter (DAT), and its phosphorylated form (ph-DAT) in DG tissue 4 h after LTP induction were quantified by western blot analysis.Monoamine reuptake and release assays were performed by using transfected HEK-293 cells.
Possible neurotoxic side effects on general behaviour were also studied.R-MO at both doses significantly enhanced spatial reference memory during the last training session and during memory retrieval compared to DMSO vehicle but not when compared to saline treated rats.Similarly, R-MO rescues DG-LTP from impairing effects of DMSO.
DMSO reduced memory BioPerine performance and LTP magnitude when compared to saline treated groups.The synaptic DR1 levels in R-MO groups were significantly decreased compared to DMSO group but were comparable with saline treated animals.We found no effect of R-MO in neurotoxicity tests.
Thus, our results support the notion that LTP-like synaptic plasticity processes could be one of the factors contributing to the cognitive enhancing effects of spatial memory traces.D1R may play an important regulatory role in these processes.